Coding

Part:BBa_K533007:Design

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)


Tar receptor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 420
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 420
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 420
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

The promoter of tar is replaced by lacI promoter and the RBS (Shine-Dalgarno sequence) is removed to reduce the expression level. As it turned out, lower level of expression ensured better chemotaxis.


Source

The sequence is cloned from the plasmid pPD12, a kind gift from Dr. Goulian.

References